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The 2nd response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as items (Fig. 9) - circular dichroism. As drawn in Fig - https://pblc.me/pub/3fc0b3e264b77b. 9, the reaction may also include electron transfer from H4B to the Fe, IIO2 intermediate


l9 As revealed in Fig. 9, this might be due to the fact that the H4B radical serve as an electron sink in a later point in the response, so as to allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.